A wheat resistosome defines common principles of immune receptor channels

Sr35L15E/L19E and AvrSr35 genes were cloned into the pFastBac1 vector (Invitrogen) with an N-terminal 6×His-SUMO tag and a glutathione S-transferase tag. Baculovirus was generally amplified to the P 2 generation before protein expression. Protein expression, purification and negative staining were analysed at EMBL Heidelberg using a Titan Krios microscope operated at 300 kV, equipped with a K3 Summit direct electron detection camera.

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