Sr35L15E/L19E and AvrSr35 genes were cloned into the pFastBac1 vector (Invitrogen) with an N-terminal 6×His-SUMO tag and a glutathione S-transferase tag. Baculovirus was generally amplified to the P 2 generation before protein expression. Protein expression, purification and negative staining were analysed at EMBL Heidelberg using a Titan Krios microscope operated at 300 kV, equipped with a K3 Summit direct electron detection camera.Read Long Article
